Journal: Archives of Medical Science : AMS

Article Title: The decrease in hippocampal transient receptor potential M2 (TRPM2) channel and muscarinic acetylcholine receptor 1 (CHRM1) is associated with memory loss in a surgical menopause rat model

doi: 10.5114/aoms.2019.83760

Figure Lengend Snippet: TRPM2 and CHRM1 gene expression levels were lower in G2 compared with G1 a Compared with G1 (p < 0.05).

Article Snippet: In order to prevent surface staining, after treatment with Ultra V Block (TA-125-UB, the Lab Vision Corporation, USA) solutions, the tissues were incubated with primary antibodies for 60 min (CHRM1 was purchased from Boster (Cholinergic receptor, muscarinic 1, catalog number: PA2202, Boster, 3942 B Valley Ave, Pleasanton, CA, 94566) and TRPM2 was purchased from Abcam (Rabbit Anti-TRPM2 antibody, ab101738, Abcam, Cambridge, UK)).

Techniques: Gene Expression

Journal: Archives of Medical Science : AMS

Article Title: The decrease in hippocampal transient receptor potential M2 (TRPM2) channel and muscarinic acetylcholine receptor 1 (CHRM1) is associated with memory loss in a surgical menopause rat model

doi: 10.5114/aoms.2019.83760

Figure Lengend Snippet: CHRM1, TRPM2 immunoreactivity and apoptotic histoscore (diffuseness × severity) and MDA levels

Article Snippet: In order to prevent surface staining, after treatment with Ultra V Block (TA-125-UB, the Lab Vision Corporation, USA) solutions, the tissues were incubated with primary antibodies for 60 min (CHRM1 was purchased from Boster (Cholinergic receptor, muscarinic 1, catalog number: PA2202, Boster, 3942 B Valley Ave, Pleasanton, CA, 94566) and TRPM2 was purchased from Abcam (Rabbit Anti-TRPM2 antibody, ab101738, Abcam, Cambridge, UK)).

Techniques: TUNEL Assay

Journal: Archives of Medical Science : AMS

Article Title: The decrease in hippocampal transient receptor potential M2 (TRPM2) channel and muscarinic acetylcholine receptor 1 (CHRM1) is associated with memory loss in a surgical menopause rat model

doi: 10.5114/aoms.2019.83760

Figure Lengend Snippet: A – Increased chrm1 immunoreactivity staining in hippocampal area is shown in G1 relative to G2 (black arrow). B – Increased trpm2 immunoreactivity staining in hippocampal area is shown in G1 relative to G2 (black arrow). C – TUNEL staining in hippocampal area is lower in G1 compared with G2 (black arrow). D – CHRM1 immunoreactivity staining in hippocampal area is lower in G2 compared with G1 (black arrow). E – TRPM2 immunoreactivity staining in hippocampal area is lower in G2 compared with G1 (black arrow). F – TUNEL staining in hippocampal area is higher in G2 compared with G1 (black arrow)

Article Snippet: In order to prevent surface staining, after treatment with Ultra V Block (TA-125-UB, the Lab Vision Corporation, USA) solutions, the tissues were incubated with primary antibodies for 60 min (CHRM1 was purchased from Boster (Cholinergic receptor, muscarinic 1, catalog number: PA2202, Boster, 3942 B Valley Ave, Pleasanton, CA, 94566) and TRPM2 was purchased from Abcam (Rabbit Anti-TRPM2 antibody, ab101738, Abcam, Cambridge, UK)).

Techniques: Staining, TUNEL Assay

Journal: Life Science Alliance

Article Title: C9ORF72 -derived poly-GA DPRs undergo endocytic uptake in iAstrocytes and spread to motor neurons

doi: 10.26508/lsa.202101276

Figure Lengend Snippet: APOJ protein levels were measured in three distinct healthy lines of iAstrocytes’ conditioned medium. (A) Regression analysis with the Four-parameter logistic curve-fit (4-PL) method was used to determine the best-fit standard curve based on twofold serial dilutions of APOJ standards and absorbance at 450 nm. (B) iAstrocytes were either left untreated or treated for 24 h with various recombinant DPRs at 1 µM (poly-GA 34 fibrils, poly-GA 34 oligomers, poly-PA 50 oligomers, and poly-GP 24 oligomers), and then APOJ protein levels in their conditioned medium were measured and calculated using the previously generated 4-PL standard curve. Bar graphs of mean ± SEM. One-way ANOVA with Tukey’s multiple-comparisons test (control group is “untreated”). * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, and **** P ≤ 0.0001.

Article Snippet: APOJ protein levels were measured using a human APOJ ELISA kit (#KE00110; ProteinTech) following the manufacturer’s instructions.

Techniques: Recombinant, Generated, Control

Journal: Cancer Science

Article Title: Immunophenotypic features of metastatic lymph node tumors to predict recurrence in N2 lung squamous cell carcinoma

doi: 10.1111/cas.12434

Figure Lengend Snippet: Univariate analysis of immunochemical staining of (a) cancer cells and (b) cancer-associated fibroblasts in primary tumors in patients with resected pathological N2 squamous cell carcinoma of the lung ( n = 64)

Article Snippet: Caveolin (clone D46G3; Cell Signaling, Danvers, MA, USA),( , ) clusterin (clone 1A11; Acris Antibodies, Herford, Germany),( , ) E-cadherin (clone 36; BD Biosciences, San Jose, CA, USA),( , ) and ZEB2 (Novus Biologicals, Littleton, CO, USA)( , ) were used as EMT-related markers.

Techniques: Staining, Variant Assay

Journal: Cancer Science

Article Title: Immunophenotypic features of metastatic lymph node tumors to predict recurrence in N2 lung squamous cell carcinoma

doi: 10.1111/cas.12434

Figure Lengend Snippet: (a) Univariate analysis of immunochemical staining of cancer cells in metastatic tumors in patients with resected pathological N2 squamous cell carcinoma of the lung ( n = 64); (b) Univariate analysis of immunochemical staining of cancer-associated fibroblasts in metastatic lymph node tumors in patients with resected pathological N2 squamous cell carcinoma of the lung

Article Snippet: Caveolin (clone D46G3; Cell Signaling, Danvers, MA, USA),( , ) clusterin (clone 1A11; Acris Antibodies, Herford, Germany),( , ) E-cadherin (clone 36; BD Biosciences, San Jose, CA, USA),( , ) and ZEB2 (Novus Biologicals, Littleton, CO, USA)( , ) were used as EMT-related markers.

Techniques: Staining, Variant Assay

Journal: Cancer Science

Article Title: Immunophenotypic features of metastatic lymph node tumors to predict recurrence in N2 lung squamous cell carcinoma

doi: 10.1111/cas.12434

Figure Lengend Snippet: Immunohistochemical staining of tumor cells in a primary tumor and a metastatic lymph node tumor in a patient with squamous cell carcinoma of the lung. (a,b) Clusterin expression of cancer cells in the primary tumor (a) and the metastatic lymph node tumor (b). (c,d) ZEB2 expression of cancer cells in the primary tumor (c) and the metastatic lymph node tumor (d). (e,f) Podoplanin expression of cancer-associated fibroblasts in the primary tumor (e) and the metastatic lymph node tumor (f). Dotted lines show the margin of the cancer cell nest.

Article Snippet: Caveolin (clone D46G3; Cell Signaling, Danvers, MA, USA),( , ) clusterin (clone 1A11; Acris Antibodies, Herford, Germany),( , ) E-cadherin (clone 36; BD Biosciences, San Jose, CA, USA),( , ) and ZEB2 (Novus Biologicals, Littleton, CO, USA)( , ) were used as EMT-related markers.

Techniques: Immunohistochemical staining, Staining, Expressing

Journal: Cancer Science

Article Title: Immunophenotypic features of metastatic lymph node tumors to predict recurrence in N2 lung squamous cell carcinoma

doi: 10.1111/cas.12434

Figure Lengend Snippet: Kaplan–Meier recurrence-free survival (RFS) curve for patients with resected pathological N2 squamous cell carcinoma of the lung according to immunohistochemical staining. (a) Kaplan–Meier RFS curve according to clusterin expression of cancer cells in metastatic lymph node tumors. (b) Kaplan–Meier RFS curve according to ZEB2 expression of cancer cells in metastatic lymph node tumors. (c) Kaplan–Meier RFS curve according to podoplanin expression of cancer-associated fibroblasts in metastatic lymph node tumors.

Article Snippet: Caveolin (clone D46G3; Cell Signaling, Danvers, MA, USA),( , ) clusterin (clone 1A11; Acris Antibodies, Herford, Germany),( , ) E-cadherin (clone 36; BD Biosciences, San Jose, CA, USA),( , ) and ZEB2 (Novus Biologicals, Littleton, CO, USA)( , ) were used as EMT-related markers.

Techniques: Immunohistochemical staining, Staining, Expressing

Journal: Cancer Science

Article Title: Immunophenotypic features of metastatic lymph node tumors to predict recurrence in N2 lung squamous cell carcinoma

doi: 10.1111/cas.12434

Figure Lengend Snippet: Multivariate analysis of clinicopathological factors for recurrence-free survival in patients with resected pathological N2 squamous cell carcinoma of the lung ( n = 64)

Article Snippet: Caveolin (clone D46G3; Cell Signaling, Danvers, MA, USA),( , ) clusterin (clone 1A11; Acris Antibodies, Herford, Germany),( , ) E-cadherin (clone 36; BD Biosciences, San Jose, CA, USA),( , ) and ZEB2 (Novus Biologicals, Littleton, CO, USA)( , ) were used as EMT-related markers.

Techniques: Expressing

Journal: Scientific Reports

Article Title: Clusterin mediates hydroquinone-induced cytotoxic responses in HL-60 differentiated cells

doi: 10.1038/s41598-024-82140-0

Figure Lengend Snippet: CLU protein concentration in HL-60 cells exposed to different concentrations of HQ. CLU protein levels were quantified using ELISA after treatment with HQ at concentrations of 0, 10, 25, and 50 µmol/L ( n = 3 per group). The error bars represent SD, and the mean is shown as horizontal bars. The control group (0 µmol/L HQ) exhibited the highest CLU concentration (19.05 ± 2.32 ng/mL), while HQ-treated groups showed a progressive decrease: 14.45 ± 1.23 ng/mL at 10 µmol/L, 9.43 ± 0.40 ng/mL at 25 µmol/L, and 9.46 ± 0.67 ng/mL at 50 µmol/L. Significant differences were determined by ANOVA followed by Tukey post hoc test (* p < 0.05, ** p < 0.01, *** p < 0.001, ns p > 0.05), ns : not significant.

Article Snippet: The concentration of sCLU proteins was measured using the Human Clusterin ELISA Kit (Boster Biological Technology, China) according to the instructions.

Techniques: Protein Concentration, Enzyme-linked Immunosorbent Assay, Control, Concentration Assay

Journal: Scientific Reports

Article Title: Clusterin mediates hydroquinone-induced cytotoxic responses in HL-60 differentiated cells

doi: 10.1038/s41598-024-82140-0

Figure Lengend Snippet: Concentration of sCLU protein in the cell culture supernatant after exposure to varying HQ concentrations. The concentration of sCLU protein in the supernatant of HL -60 cell cultures was determined using ELISA following treatment with HQ at 0, 10, 25, and 50 µmol/L ( n = 3 per group). The control group (0 µmol/L HQ) exhibited the highest concentration (25.88 ± 4.24 ng/mL), while the 10 µmol/L HQ group showed a slight, non-significant decrease (23.73 ± 2.03 ng/mL, p = 0.696). Significant reductions were observed in the 25 µmol/L (17.45 ± 0.36 ng/mL) and 50 µmol/L (12.96 ± 0.67 ng/mL) groups compared to the control (* p < 0.05, *** p < 0.001, ns p > 0.05), ns : not significant.

Article Snippet: The concentration of sCLU proteins was measured using the Human Clusterin ELISA Kit (Boster Biological Technology, China) according to the instructions.

Techniques: Concentration Assay, Cell Culture, Enzyme-linked Immunosorbent Assay, Control